Calcium-binding protein and vitamin D metabolism in experimental protein malnutrition

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Vitamin D-dependent Calcium-binding Protein

A method of purification of a vitamin D-dependent calciumbinding protein from chick intestinal mucosa is described. The purification procedure involved the use of ammonium sulfate precipitation, gel filtration on Sephadex G-100, and preparative disc electrophoresis. The assay system for calcium-binding protein is based upon the competition between calcium-binding protein and an ion exchange res...

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Vitamin D Binding Protein and Vitamin D Levels

1 Institute of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China 2 Clinical Research, Diabetes and Endocrinology, Merck Research Laboratories, 126 East Lincoln Avenue, RY34B-148, Rahway, NJ 07065-0900, USA 3Department of Nutritional Science, Rutgers University, New Brunswick, NJ 08901, USA 4Division of Endocrinology, Metabolism an...

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Placental calcium binding protein in rats. Apparent identity with vitamin D-dependent calcium binding protein from rat intestine.

Rat placenta contains a calcium binding protein (CaBP) which is closely related or identical with the vitamin Ddependent CaBP in rat intestine. Placental CaBP was detected using antiserum prepared in rabbits against the intestinal form of the protein purified as previously described (Bruns, M. E. H., Fliesher, E. B., and Avioli, L. V. (1977) J. Biol. Chem. 252, 4145-4150). The antiserum was mon...

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Calcium and Vitamin D Metabolism in Pediatric Nephrotic Syndrome; An Update on the Existing Literature

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The relationship between vitamin D-stimulated calcium transport and intestinal calcium-binding protein in the chicken.

1. The rapid stimulation of intestinal Ca(2+) transport observed in vitamin D-deficient chicks after receiving 1,25-dihydroxycholecalciferol has necessitated a re-evaluation of the correlation hitherto observed between this stimulation and the induction of calcium-binding protein synthesis. By 1h after a dose of 125ng of 1,25-dihydroxycholecalciferol, Ca(2+) transport is increased. This is at l...

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ژورنال

عنوان ژورنال: British Journal of Nutrition

سال: 1974

ISSN: 0007-1145,1475-2662

DOI: 10.1079/bjn19740110